Hybrid volumetric calcium imaging

Volumetric Medical Imaging Environment

We describe a graphical tool for analysis and visualization of three dimensional medical data. The application is intended to help physicians interpret the geometric structures of volumetric medical data and navigate through it easily. It combines novel approaches for segmenting the data and advanced techniques for visualizing the models which allow fast processing on a standard personal comput...

Volumetric imaging of fish locomotion.

Fishes use multiple flexible fins in order to move and maintain stability in a complex fluid environment. We used a new approach, a volumetric velocimetry imaging system, to provide the first instantaneous three-dimensional views of wake structures as they are produced by freely swimming fishes. This new technology allowed us to demonstrate conclusively the linked ring vortex wake pattern that ...

Volumetric determination of calcium in blood serum.

Organization of vomeronasal sensory coding revealed by fast volumetric calcium imaging.

A long-standing goal in neuroscience is to perform exhaustive recording of each neuron in a functional local circuit. To achieve this goal, one promising approach is optical imaging of fluorescent calcium indicators, but typically the tens or hundreds of cells imaged simultaneously comprise only a tiny percentage of the neurons in an intact circuit. Here, we show that a recent innovation, objec...

Hybrid Multiphoton Volumetric Functional Imaging of Large Scale Bioengineered Neuronal Networks

Planar neural networks and interfaces serve as versatile in vitro models of central nervous system physiology, but adaptations of related methods to three dimensions (3D) have met with limited success. Here, we demonstrate for the first time volumetric functional imaging in a bioengineered neural tissue growing in a transparent hydrogel with cortical cellular and synaptic densities, by introduc...